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recombinant human fgf4 rhfgf4  (R&D Systems)


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    R&D Systems recombinant human fgf4 rhfgf4
    Recombinant Human Fgf4 Rhfgf4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 170 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+fgf4+rhfgf4/Recombinant+Human+FGF-4+Protein/pmc10280139-107-34-38
    Average 95 stars, based on 170 article reviews
    recombinant human fgf4 rhfgf4 - by Bioz Stars, 2026-10
    95/100 stars

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    Cell Culture:

    Article Title: A chemically defined system supports two distinct types of stem cell from a single blastocyst and their self‐assembly to generate blastoid
    Article Snippet: On day 6–7, the outgrowth was retrieved, dissociated with 0.05% trypsin–EDTA (Biological Industries), and plated into a new 24‐well plate with MEF feeders. .. TSCs were cultured in TSCs basal medium composed of RPMI 1640 (Gibco) supplemented with 20% foetal bovine serum (FBS) (Gibco), 1× GlutaMAX (Gibco), 1× Sodium pyruvate (Gibco), and 0.1 mM 2‐mercaptoethanol (Sigma), 25 ng/ml recombinant human FGF4 (rhFGF4) (R&D Systems) and 1 μg/ml Heparin (Sigma‐Aldrich). ..

    Recombinant:

    Article Title: A chemically defined system supports two distinct types of stem cell from a single blastocyst and their self‐assembly to generate blastoid
    Article Snippet: On day 6–7, the outgrowth was retrieved, dissociated with 0.05% trypsin–EDTA (Biological Industries), and plated into a new 24‐well plate with MEF feeders. .. TSCs were cultured in TSCs basal medium composed of RPMI 1640 (Gibco) supplemented with 20% foetal bovine serum (FBS) (Gibco), 1× GlutaMAX (Gibco), 1× Sodium pyruvate (Gibco), and 0.1 mM 2‐mercaptoethanol (Sigma), 25 ng/ml recombinant human FGF4 (rhFGF4) (R&D Systems) and 1 μg/ml Heparin (Sigma‐Aldrich). ..



    Similar Products

    recombinant human fgf4 rhfgf4  (R&D Systems)
    95
    R&D Systems recombinant human fgf4 rhfgf4
    Recombinant Human Fgf4 Rhfgf4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+fgf4+rhfgf4/Recombinant+Human+FGF-4+Protein/pmc10280139-107-34-38
    Average 95 stars, based on 1 article reviews
    recombinant human fgf4 rhfgf4 - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier
    recombinant human fgf4 rhfgf4  (PeproTech)
    90
    PeproTech recombinant human fgf4 rhfgf4
    A. Brightfield (right panels) and Venus-fluorescence (middle) in Spry4H2B-Venus ESCs grown in serum/LIF medium (upper panels) or serum/LIF supplemented with 1 μM PD0325901 for 3 days (lower panels). Scale bar, 25 μm. B. Schematic diagram depicting experimental approach to determine ERK responsiveness of the reporter. Cells were cultured for 3 days in serum/LIF conditions supplemented with 1 μM PD0325901 to downregulate reporter expression, followed by transfer into subsaturating PD03 concentrations for one day. C. Flow cytometry analysis of Venus expression in Spry4H2B-Venus ESCs treated as indicated in (B). Uppermost histogram is from the Spry4 wild-type parental cell line. D. Quantification of results from (C). Expression of Venus in cells maintained in serum/LIF medium was normalized to 1, data points show average +/− SD from four independent experiments. E. Schematic of experimental approach to determine FGF responsiveness of the reporter. Wild-type and Fgf4 mutant cells were cultured in 2i/LIF conditions for three days followed by transfer into N2B27 medium supplemented with various doses of FGF4 or <t>FGF2</t> for one day, or no FGF for parental, wild-type and mutant controls. F. Flow cytometry analysis of Venus expression in Fgf4 wild-type (wt) and Fgf4−/− mutant Spry4H2B-Venus ESCs treated as indicated in (E). The level of Venus expression in Fgf4 mutant cells is comparable to wt parental ESCs without the reporter, indicating that reporter expression in Fgf4 wt cells is mainly triggered by ESC-secreted FGF4. N = 3, one representative experiment shown. G. Total ERK and ppERK levels in Fgf4 mutant cells treated as indicated in (E) determined by immunoblotting. N = 3, one representative experiment shown. H. Relationship between ERK phosphorylation and Venus expression in Fgf4−/− mutant ESCs treated with different doses of FGF4 and FGF2 compiled from data in panels F and G. Data normalized to ppERK and Venus levels in cells treated with the highest dose of FGF4 (shown as 1). Data points indicate average +/− SD from two (Fgf4 mutant, 0 ng/ml FGF) or three (all other data points) independent experiments.
    Recombinant Human Fgf4 Rhfgf4, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+fgf4+rhfgf4/fibroblast+growth+factor+4/pmc06455974-535-12-19
    Average 90 stars, based on 1 article reviews
    recombinant human fgf4 rhfgf4 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

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    A. Brightfield (right panels) and Venus-fluorescence (middle) in Spry4H2B-Venus ESCs grown in serum/LIF medium (upper panels) or serum/LIF supplemented with 1 μM PD0325901 for 3 days (lower panels). Scale bar, 25 μm. B. Schematic diagram depicting experimental approach to determine ERK responsiveness of the reporter. Cells were cultured for 3 days in serum/LIF conditions supplemented with 1 μM PD0325901 to downregulate reporter expression, followed by transfer into subsaturating PD03 concentrations for one day. C. Flow cytometry analysis of Venus expression in Spry4H2B-Venus ESCs treated as indicated in (B). Uppermost histogram is from the Spry4 wild-type parental cell line. D. Quantification of results from (C). Expression of Venus in cells maintained in serum/LIF medium was normalized to 1, data points show average +/− SD from four independent experiments. E. Schematic of experimental approach to determine FGF responsiveness of the reporter. Wild-type and Fgf4 mutant cells were cultured in 2i/LIF conditions for three days followed by transfer into N2B27 medium supplemented with various doses of FGF4 or FGF2 for one day, or no FGF for parental, wild-type and mutant controls. F. Flow cytometry analysis of Venus expression in Fgf4 wild-type (wt) and Fgf4−/− mutant Spry4H2B-Venus ESCs treated as indicated in (E). The level of Venus expression in Fgf4 mutant cells is comparable to wt parental ESCs without the reporter, indicating that reporter expression in Fgf4 wt cells is mainly triggered by ESC-secreted FGF4. N = 3, one representative experiment shown. G. Total ERK and ppERK levels in Fgf4 mutant cells treated as indicated in (E) determined by immunoblotting. N = 3, one representative experiment shown. H. Relationship between ERK phosphorylation and Venus expression in Fgf4−/− mutant ESCs treated with different doses of FGF4 and FGF2 compiled from data in panels F and G. Data normalized to ppERK and Venus levels in cells treated with the highest dose of FGF4 (shown as 1). Data points indicate average +/− SD from two (Fgf4 mutant, 0 ng/ml FGF) or three (all other data points) independent experiments.

    Journal: Developmental biology

    Article Title: A Sprouty4 reporter to monitor FGF/ERK signaling activity in ESCs and mice

    doi: 10.1016/j.ydbio.2018.06.017

    Figure Lengend Snippet: A. Brightfield (right panels) and Venus-fluorescence (middle) in Spry4H2B-Venus ESCs grown in serum/LIF medium (upper panels) or serum/LIF supplemented with 1 μM PD0325901 for 3 days (lower panels). Scale bar, 25 μm. B. Schematic diagram depicting experimental approach to determine ERK responsiveness of the reporter. Cells were cultured for 3 days in serum/LIF conditions supplemented with 1 μM PD0325901 to downregulate reporter expression, followed by transfer into subsaturating PD03 concentrations for one day. C. Flow cytometry analysis of Venus expression in Spry4H2B-Venus ESCs treated as indicated in (B). Uppermost histogram is from the Spry4 wild-type parental cell line. D. Quantification of results from (C). Expression of Venus in cells maintained in serum/LIF medium was normalized to 1, data points show average +/− SD from four independent experiments. E. Schematic of experimental approach to determine FGF responsiveness of the reporter. Wild-type and Fgf4 mutant cells were cultured in 2i/LIF conditions for three days followed by transfer into N2B27 medium supplemented with various doses of FGF4 or FGF2 for one day, or no FGF for parental, wild-type and mutant controls. F. Flow cytometry analysis of Venus expression in Fgf4 wild-type (wt) and Fgf4−/− mutant Spry4H2B-Venus ESCs treated as indicated in (E). The level of Venus expression in Fgf4 mutant cells is comparable to wt parental ESCs without the reporter, indicating that reporter expression in Fgf4 wt cells is mainly triggered by ESC-secreted FGF4. N = 3, one representative experiment shown. G. Total ERK and ppERK levels in Fgf4 mutant cells treated as indicated in (E) determined by immunoblotting. N = 3, one representative experiment shown. H. Relationship between ERK phosphorylation and Venus expression in Fgf4−/− mutant ESCs treated with different doses of FGF4 and FGF2 compiled from data in panels F and G. Data normalized to ppERK and Venus levels in cells treated with the highest dose of FGF4 (shown as 1). Data points indicate average +/− SD from two (Fgf4 mutant, 0 ng/ml FGF) or three (all other data points) independent experiments.

    Article Snippet: For activation of the FGF/ERK pathway in ESCs, 10–100 ng/ml of recombinant human FGF2 (rhFGF2, CellGS) or FGF4 (rhFGF4, Peprotech) was added to the specified culture medium with 1 μg/ml of Heparin Sulfate (Sigma).

    Techniques: Fluorescence, Cell Culture, Expressing, Flow Cytometry, Mutagenesis, Western Blot, Phospho-proteomics